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Test Mnemonic GCRNA Neisseria gonorrhoeae by Nucleic Acid Amplification (GEN-PROBE)

Reporting Name

Neisseria gonorrhoeae Amplified RNA

Useful For

Detection of Neisseria gonorrhoeae

Performing Laboratory

Mayo Medical Laboratories in Rochester

Specimen Type

Varies


Necessary Information


Specimen source is required.



Specimen Required


Submit only 1 of the following specimens:

 

Swab specimen must be collected using an APTIMA Collection Unisex Swab (T583) or APTIMA Collection Vaginal Swab (T584). These swabs are contained in the APTIMA Collection Kit.

 

Supplies: Swab, Aptima Male/Female Collection (T583)

Specimen Type: Endocervix

Container/Tube: APTIMA Collection Unisex Swab (T583)

Specimen Volume: Swab

Collection Instructions:

1. Use cleaning swab (white shaft) to remove excess mucus from endocervix and discard.

2. Insert second swab (blue shaft) 1 to 1.5 cm into endocervical canal, and rotate swab gently for 30 seconds. Avoid touching vaginal wall when removing swab.

3. Place second swab into transport tube provided in collection kit. Snap off swab at score line so swab fits into closed tube.

4. Cap tube securely, and label tube with patient's entire name, and date and time of collection.

5. Transport and store swab container at 2 to 30° C (refrigerate is preferred temperature) within 60 days of collection. If longer storage is needed, freeze at -20 to -70° C for 12 months.

 

Supplies: Swab, Aptima Vaginal Swab Specimen Collection Kit (T584)

Specimen Type: Vaginal

Container/Tube: APTIMA Collection Vaginal Swab (T584)

Specimen Volume: Swab

Collection Instructions:

1. Insert swab (pink shaft) about 5 cm past introitus and rotate gently for 30 seconds.

2. Place swab into transport tube provided in collection kit. Snap off swab at score line so swab fits into closed tube.

3. Cap tube securely, and label tube with patient's entire name, and date and time of collection.

4. Transport and store swab container at 2 to 30° C (refrigerate is preferred temperature) within 60 days of collection. If longer storage is needed, freeze at -20 to -70° C for 12 months.

 

Supplies: Swab, Aptima Male/Female Collection (T583)

Specimen Type: Urethra (Males Only)

Container/Tube: APTIMA Collection Unisex Swab (T583)

Specimen Volume: Swab

Collection Instructions:

1. Patient should not have urinated for at least 1 hour prior to collection.

2. With a rotating movement, insert swab (blue shaft) 2 to 4 cm into urethra.

3. Once inserted, rotate swab gently at least 1 full rotation using sufficient pressure to ensure swab comes into contact with all urethral surfaces. Allow swab to remain inserted for 2 to 3 seconds.

4. Place swab in transport tube provided in collection kit. Snap off swab at score line so swab fits into closed tube.

5. Cap tube securely, and label tube with patient's entire name, and date and time of collection.

6. Transport and store swab container at 2 to 30° C (refrigerate is preferred temperature) within 60 days of collection. If longer storage is needed, freeze at -20 to -70° C for 12 months.

 

Supplies: Aptima Urine Transport Tube (T582)

Specimen Type: Urine (Males and Females)

Container/Tube: APTIMA Urine Specimen Transport Tube (T582)

Specimen Volume: 15-20 mL

Collection Instructions:

1. Patient should not have urinated for at least 1 hour prior to specimen collection.

2. Patient should collect first portion of random voided urine (first part of stream) into a sterile, plastic, preservative-free container.

3. Transfer 2 mL of urine into the urine specimen transport tube using the disposable pipette provided within 24 hours of collection. The correct volume of urine has been added when the fluid level is between the black fill lines on the urine transport tube. Place the labels on the transport tube so the black fill lines are still visible for volume confirmation at Mayo Medical Laboratories.

4. Transport and store urine specimen transport container at 2 to 30° C (refrigerate is preferred temperature) within 30 days of collection. If longer storage is needed, freeze at -20 to -70° C for 12 months.

 

Supplies: Aptima Thin Prep Transport Tube (T652)

Specimen Type: ThinPrep Specimen (Endocervix)

Container/Tube: ThinPrep (also called PreservCyt) Collection Kit (T652)

Specimen Volume: 1 mL

Collection Instructions:

1. Aliquot ThinPrep specimen for Chlamydia and/or Neisseria testing before processing for Pap smear. For each specimen, use a new pair of clean gloves.

2. Vortex ThinPrep/PreservCyt vial 3 to 10 seconds. Within 1 minute of vortexing:

a. Transfer 1 mL of specimen into the APTIMA Specimen Transfer Tube (T652) using a disposable transfer pipette or a pipette tip containing a filter (aerosol barrier or hydrophobic plug).

b. Process only 1 ThinPrep and transfer tube set at a time.

c. Recap APTIMA Specimen Transfer Tube tightly and gently invert 3 times to mix.

3. Label APTIMA transfer tube with appropriate label.

4. Use remainder of ThinPrep specimen for Pap testing.

5. Transport and store specimen transfer container at 2 to 30° C (refrigerate is preferred temperature) within 60 days of collection. If longer storage is needed, freeze at -20 to -70° C for 12 months.


Specimen Minimum Volume

Urine: 2 mL

Specimen Stability Information

Specimen Type Temperature Time
Varies Refrigerated (preferred)
  Ambient 
  Frozen 

Reference Values

Negative

Day(s) and Time(s) Performed

Monday through Saturday; Varies

Test Classification

This test has been cleared or approved by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

87591

LOINC Code Information

Test ID Test Order Name Order LOINC Value
GCRNA Neisseria gonorrhoeae Amplified RNA 43305-2

 

Result ID Test Result Name Result LOINC Value
SRC17 SOURCE: 31208-2
34875 Neisseria gonorrhoeae amplified RNA 43305-2

Method Name

Transcription Mediated Amplification

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request Form (T244) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/microbiology_test_request_form.pdf).