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Test Mnemonic TCGRV T-Cell Receptor Gene Rearrangement, PCR, Varies

Reporting Name

T Cell Receptor Gene Rearrange, V

Useful For

Determining whether a T-cell population is polyclonal or monoclonal

Performing Laboratory

Mayo Medical Laboratories in Rochester

Specimen Type

Varies


Shipping Instructions


Body fluid or spinal fluid specimens must arrive within 4 days (96 hours) of collection.



Specimen Required


Submit only 1 of the following specimens:

 

Specimen Type: Body fluid

Container/Tube: Sterile container

Specimen Volume: At least 5 mL

Collection Instructions:

1. If the volume is large, pellet cells prior to sending.

2. Send less volume at ambient temperature or as a frozen cell pellet.

Specimen Stability Information:

Body fluid: Ambient/Refrigerated/Frozen

Cell pellet: Frozen

 

Specimen Type: Paraffin-embedded bone marrow aspirate clot

Container/Tube: Paraffin block

Specimen Stability Information: Ambient/Refrigerated

 

Specimen Type: Frozen tissue

Container/Tube: Plastic container

Specimen Volume: 100 mg

Collection Instructions: Freeze tissue within 1 hour of collection.

Specimen Stability Information: Frozen

 

Specimen Type: Paraffin-embedded tissue

Container/Tube: Paraffin block

Specimen Stability Information: Ambient/Refrigerated/Frozen

 

Specimen Type: Spinal fluid

Container/Tube: Sterile vial

Specimen Volume: 5-10 mL

Specimen Stability Information: Ambient/Refrigerated

 

Specimen Type: Extracted DNA from blood or bone marrow

Container/Tube: 1.5- to 2-mL tube with indication of volume and concentration of DNA

Specimen Volume: Entire specimen

Collection Instructions: Label specimen as extracted DNA from blood or bone marrow

Specimen Stability Information: Refrigerated/Ambient


Specimen Minimum Volume

Body Fluid or Spinal Fluid: 1 mL; Tissue: 50 mg; Extracted DNA from Blood or Bone Marrow: 50 microliter at 20 ng/microliter

Specimen Stability Information

Specimen Type Temperature Time
Varies Varies

Reference Values

An interpretive report will be provided.

Positive, negative, or indeterminate for a clonal T-cell population

Day(s) and Time(s) Performed

Monday through Friday

Test Classification

This test was developed using an analyte specific reagent. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

81340-TCB (T cell antigen receptor, beta) (eg, leukemia and lymphoma), gene rearrangement analysis to detect abnormal clonal population(s); using amplification methodology (eg, PCR)

81342-TCG@ (T cell receptor, gamma) (eg, leukemia and lymphoma), gene rearrangement analysis, evaluation to detect abnormal clonal population(s)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
TCGRV T Cell Receptor Gene Rearrange, V In Process

 

Result ID Test Result Name Result LOINC Value
19936 Final Diagnosis: 34574-4
MP016 Specimen: 31208-2

Method Name

DNA Extracted for Analysis/Polymerase Chain Reaction (PCR)

Forms

1. Hematopathology Patient Information (T676) in Special Instructions

2. If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request Form (T726) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/hematopathology-request-form.pdf)